Cognition, Behavior, and Memory
Author: Jennifer Luciana Miranda | email: jenniferlucianamiranda@gmail.com
Jennifer Miranda 1°, Erika Georgieff 2°, Ariel Martínez Dorr 1°, Eduardo Cánepa 2°, Mariela Chertoff 2°
1° Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica, Laboratorio de Neuroepigenética y adversidades tempranas. Buenos Aires, Argentina.
2° Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica, Laboratorio de Neuroepigenética y adversidades tempranas. Buenos Aires, Argentina. CONICET- Universidad de Buenos Aires. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. (IQUIBICEN). Buenos Aires, Argentina.
Several studies have shown that maternal stress alters the brain maturation of the embryo, resulting in the modification of cognitive and socio-emotional functions. However, not all individuals deal on the same way to stress. In order to analyze the mechanisms of resilience to stress, we are seting up a mice model of gestational restraint in which, pregnant CF1 adult females were subjected to movement restriction for 45 min, three times per day, from GD10 to GD19. GD0 was set on the day of vaginal plug observed. Weight of dams and pups were controlled. As maternal behavior might varied by stress, it was evaluated each 3 minutes from PD1 to PD5 at 9 am for 1 hour. After weaning, anhedonia and motivation in the dams were evaluated with the splash test. In addition, in order to separate between resilient and susceptible mice, all males and females pups were subjected to Splash test at 5 weeks of age. In both cases, the evaluation consisted on one first splash followed by 45 min of restraint and then other 5 min splash test. Grooming time was evaluated in each test. We proposed that resilient mice behaved as control mice in the splash test and susceptible mice performed less grooming behavior as consequence of less motivation. Other behaviors related with anxiety and depression were analyzed in the three different groups: control and resilient and vulnerable mice (last two born from restricted dams).